Regina started out as the kind-hearted daughter of Cora Mills and Henry Mills I. She was in love with the stable boy, Daniel, and was torn between her love for him and her love for her mother. One day she saved Snow White, and Snow's father proposed to her. She planned on running away, but Snow found out and eventually told Cora, who had tricked Snow. Cora ripped Daniel's heart out and crushed it into dust, and since then Regina's hatred for Snow White had grown into a desire to kill her.
Regina first appears as the evil queen who interrupts the wedding between Snow White and Prince Charming. She claims that her gift to them is their wedding day, for tomorrow her real work begins. Regina threatens to tear everyone's happiness away, especially Snow White's. As she turns to leave, Charming attempts to kill her but she vanishes into smoke. She reappears at her castle, where her father offers her a drink. Regina rudely remarks, "Do I look like I need a drink?" to which he replies that he was only trying to help. She takes the drink and thanks him, before the Magic Mirror appears and asks her how she is going to destroy everybody's happiness. Regina reveals that she is going to use the Dark Curse, which concerns both Henry and the Magic Mirror. She visits Maleficent and after a brief battle with her, manages to obtain the Dark Curse. Next, she hosts a meeting with the darkest souls in the land, requiring a strand of each of their hair in order to activate the curse. Lastly, she sacrifices the heart of her childhood steed, but this doesn't work. Her father suggests that she should go and question the man who created the Dark Curse: Rumplestiltskin. She visits him, and he tells her that the heart of a horse won't do, she needs the heart of the thing she loves most. Regina realises that she needs the heart of her father, and tearfully performs this. She throws his heart into a fire, which activates the Dark Curse. Regina takes the carriage to Snow White and Prince Charming's castle, and has enough time to gloat over Snow and reveal that this is her happy ending, before they are all consumed by the curse.
- S1, E1: "Pilot"
- S1, E2: "The Thing You Love Most"